Enhanced eDNA Detection
- Our TripleLock™Platform yields the best environmental DNA qPCR assays available. Our expanding portfolio of advanced TripleLock ™ qPCR tests are held to rigorous performance standards, then validated by a third party ISO17025 accredited laboratory facility.
- Get the best value – we offer water sampling, eDNA extraction and test results in less than 80 minutes saving you time and money.
- Our expanding portfolio of advanced TripleLock ™ tests are held to rigorous performance standards.
- We continuously invest in developing new solutions, so you’re equipped to meet the environmental challenges of today and the future.
Platform Core I. Gold-Standard TripleLock™ qPCR Assays
It all begins with having the best, most reliable qPCR Assays. At Precision Biomonitoring, we develop Gold-Standard Environmental DNA Assays that exceed the Minimal Mandatory qPCR Standards for Publication (MIQE Guidelines 2009). Our assays are rigorously developed first in silico, then lab validated using target DNA and DNA of closely related organisms, to ensure that the assay is truly specific to the target species.
We don’t stop at that. Once optimized and validated in our facilities, we send our assays to an ISO 17025 accredited laboratory for a “second opinion.” This facility verifies that our assays are extremely efficient and sensitive, with detection limits below 10 copies of eDNA per reaction.
When third party validation is complete, we manufacture test kits for field use. During this process, our primers, probes, and qPCR mastermix is lyophilized and prepared into test strips containing an Internal Positive Control.The test strips are thermostable, and ready for field use or in-house qPCR.
The last step in our assay development is Field Validation. During field validation, we test our assays in known positive and negative sites. This means going to habitats where there are known populations of the target species to verify assay performance. We also do this in confirmed negative sites, where the target species is not present (but closely related species may be), to verify that our assay is truly specific in our lab, in a third party lab, and in the field where it matters most.
Platform Core II. Optimal Survey Design
Survey design is amongst the most important considerations in eDNA sampling. It is simply not enough to take low-volume surface water sample and infer that a target species is present or absent from a particular site. At Precision Biomonitoring Inc, we work with you to develop and optimize your eDNA survey.
We begin by getting to the root of your eDNA survey. What question do you seek to answer? Some questions are easier to answer than others, and some questions may require significant sampling intensity to answer with confidence. In all cases, our surveys are designed based on advanced statistics to maximize confidence in results.
Next, we consider the target species’ biology and ecology. Not all species share the same biological activity. Metabolic activity and DNA shedding rates can vary significantly between species, and this must be accounted for in survey design. Furthermore, we consider the target species’ ecology and behavioural patterns to maximize the probability of eDNA detection. This means sampling where the species should be based on time of day, season, and a number of other key factors.
We pair the species biological and ecological information with site specific metadata, taking into account site details such as flow rates, turbidity, temperature, depth, and many other factors to optimize our sampling strategies.
For deepwater species, we use cutting edge sonar technology to find the structures and conditions that our target species prefer, then collect our samples directly from these areas at select depths.
Upon collection of these data through site research and a small pilot study, we get to work on designing the optimal eDNA survey for the target species in a given water body. All of this is done using our proprietary survey design software (patent pending).
Platform Core III. On-Site Detection
At Precision Biomonitoring, we are committed to delivering accurate results, fast. In molecular biology, rapid production of results can be tough. That is why we have teamed up with Smith-Root and Biomeme to amalgamate the previously separated processes of sample collection, water filtration, DNA extraction, and qPCR analysis.
The advantages of this system are clear. We can sample very large volumes of water quickly from highly specific locations, and immediately extract eDNA from our samples. This ensures that eDNA samples are not degraded during transit, and are not exposed to contaminants often found in central labs.
Using the sensor integrated water filtration system ANDe™, developed by Smith-Root, we filter water samples on-site. This “smart pump” enables the user to modify target sampling volume, pump pressure limits, and flow rate limits using on-board controls. These variables can have a significant influence on the quantity of environmental DNA that is captured by the single-use nitrocellulose filters used during sampling.
Following sample collection, the nitrocellulose filter membrane is removed from the filter cartridge and placed into a Lysis Buffer to split apart captured cells and to remove collected eDNA from the filter in to solution. After a series of wash steps, the eDNA is purified and transferred into a thermostable, elution buffer. This process is complete in a matter of minutes, and produces high quality purified nucleic acids. All without requiring a vortexer, heatblock, or centrifuge. The eluted sample is then ready for qPCR analysis.
On-Site qPCR: Biomeme Three9™
The Biomeme Three9™is a powerful handheld, ultra portable device that enables PCR, RT-PCR, qPCR, and Isothermal tests.
In a single 30-60 minute run, the 9-well and 3-fluorescent channel Three9™can simultaneously test up to 9 samples, or 27 targets.
Mobile Data Management System
- The Biomeme mobile app captures test results and uploads them directly to the Biomeme cloud for immediate access and management
- Intuitive phone interface with on-screen tutorials
- Program your custom thermocycling parameters and view raw test data
- Run tests offline and sync data to the Cloud Portal via onboard Wifi/Cellular
- View GPS-tagged results in real-time
- Manage data from multiple Three9 devices and experiments in one place
- View PCR amplification plots, GPS metadata, and more
- Download raw data files for easy analysis
- Build custom apps and integrations using the API