TripleLock TM
FASTER RESPONSE TIME BY DESIGN
Our platform is based on three core principles that are designed to maximize accuracy and confidence in qPCR results, reduce costs, and deliver results in real-time.

āŗāŗāŗ The best qPCR assays available. Our expanding portfolio of advanced TripleLock ⢠qPCR tests are held to rigorous performance standards, then validated by a third party ISO17025 accredited laboratory facility.
āŗāŗāŗ Get the best value ā Onsite DNA and RNA extraction in 3-5 minutes
āŗāŗāŗ Onsite accurate qPCRĀ test results in less than 60 minutes saving you time and money.
1
Gold-standard TripleLock TM qPCR Assays
It all begins with having the best, most reliable qPCR Assays. At Precision Biomonitoring, we develop Gold-Standard DNA Assays that exceed the Minimal Mandatory qPCR Standards for Publication (MIQE Guidelines 2009). The assays are rigorously developed firstĀ inĀ silico,Ā then lab validated using target DNA and DNA of closely related organisms, to ensure that the assay is truly specific to the target species. We have developed and validated 60 assays so far.

The last step in our assay development is Field Validation. During field validation, we test our assays in known positive and negative sites. This means going to locations where there are known populations of the target species to verify assay performance. We also do this in confirmed negative sites, where the target species is not present (but closely related species may be), to verify that our assay is truly specific in our lab, in a third party lab, and in the field where it matters most.
2
Onsite DNA or RNA Extraction in 3 Minutes
Following sample collection, the sample is placed into a Lysis Buffer to split apart captured cells and to remove collected DNA or RNA from the filter into solution. After a series of wash steps, the eDNA is purified and transferred into a thermostable, elution buffer. This process is complete in 3 minutes, and produces room-stable high quality purified nucleic acids. All this without requiring a vortexer, heatblock, or centrifuge. The eluted sample is then ready for qPCR analysis.

3
On-site qPCR: Biomeme Three9

The Biomeme Three9ā¢is a powerful handheld, ultra portable device that enables PCR, RT-PCR, qPCR, and Isothermal tests. In a single 30-60 minute run, the 9-well and 3-fluorescent channel Three9ā¢can simultaneously test up to 9 samples, or 27 targets.
4
Mobile Data Management System
āŗāŗāŗ The Biomeme mobile app captures test results and uploads them directly to the Biomeme cloud for immediate access and management
āŗāŗāŗ Intuitive phone interface with on-screen tutorials
āŗāŗāŗ Program your custom thermocycling parameters and view raw test data
āŗāŗāŗ Run tests offline and sync data to the Cloud Portal via onboard Wifi/Cellular
āŗāŗāŗ View GPS-tagged results in real-time
āŗāŗāŗ Manage data from multiple Three9 devices and experiments in one place
āŗāŗāŗ View PCR amplification plots, GPS metadata, and more
āŗāŗāŗ Download raw data files for easy analysis
āŗāŗāŗ Build custom apps and integrations using the API

The TripleLock Platform is adaptable, and can be customized to detect any target organism. Think our platform could be adapted to your business?Ā Weād love to chat!
info@precisionbiomonitoring.com
1-888-444-7702
Office Address:
Orchard ParkĀ – Suite #226Ā
5420 Highway 6 North
Guelph ONĀ Ā Canada
N1H 6J2
Lab Address:
361 Southgate Drive
Guelph ON Canada
N1G 3M5