Category: eDNA

Exploring MIQE-Like Standards for qPCR-Based Molecular Detection of eDNA

In the previous blog, we briefly touched on the MIQE standards – guidelines published to promote minimal standards of reporting qPCR data from clinical trials,  used to detect small relative changes in gene expression within cells or tissues, or to quantify the amount of pathogen(s). As much of contemporary environmental DNA work is conducted using…

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Detecting eDNA – The Importance of Assay Specificity and Sensitivity Part I: An Introduction to the MIQE Guidelines and DNA Sequence Database Generation & Curation for qPCR Assay Design

on Using Environmental DNA (eDNA) to Monitor Aquatic Ecosystems MIQE – a Brief Introduction In 2009, Bustin and colleagues[1] codified a set of minimal requirements for the publication of quantitative real-time polymerase chain reaction (qPCR) data, ostensibly for use in the fields of clinical medicine and molecular biology, as a means of ensuring rigorous datasets that enable…

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Using Environmental DNA (eDNA) to Monitor Aquatic Ecosystems

Environmental DNA monitoring – eDNA – is at the vanguard of a new wave of technologically advanced monitoring efforts. With roots in soil and paleoecology, eDNA was first used to detect a multicellular aquatic organism– the invasive American bullfrog Lithobates catesbeianus– in a landmark scientific paper by Ficetola et al. in 2008[1]. By applying the widely used,…

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